Synthesis of a new diarylhydrazone derivative and an evaluation of its in vitro biofilm inhibition and quorum sensing disruption along with a molecular docking study

Molecules that target quorum sensing and biofilm inhibition are useful antimicrobials. In this regard, a new diarylhydrazone was synthesized characterized using infrared, high-resolution mass spectrometry nuclear magnetic resonance experiments as N-[( E)-4-bromo-2,5-diheptyloxybenzylideneamino]-2,4-dinitroaniline (BHBANA). Minimal inhibitory concentrations (MICs) vary from 0.625 to 2.5 mg mL −1 . This compound screened in vitro for its of sensing–mediated violacein production by Chromobacterium violaceum CV12472 at MIC sub-MIC showed percentage varying 100% 5.7% ± 0.2% MIC/32. Against CV026, BHBANA exhibited anti-quorum-sensing zone diameters 10.5 0.3 mm 7.0 0.1 MIC/2, respectively. shows concentration-dependent swarming motility on flagellated Pseudomonas aeruginosa PA01 with the highest % 28.30% 0.50% μg MIC. The product inhibits formation, best being observed against Staphylococcus aureus 72.24% 0.86% (MIC) 09.82% 0.10% (MIC/8). Molecular docking studies carried out utilizing Schrodinger software identified interactions between different receptor compartments violaceum, which can block pathogenic gene expression. results suggest potential reducing microbial virulence.